李崇华,赵方慈,喻琪盛,陈杨沁,张春华,葛滢.胞外聚合物对莱茵衣藻砷富集和形态转化的影响[J].湖南农业大学学报:自然科学版,2019,45(4):.
胞外聚合物对莱茵衣藻砷富集和形态转化的影响
  
DOI:
中文关键词:  莱茵衣藻  胞外聚合物    富集  转化
英文关键词:Chlamydomonas reinhardtii  extracellular polymeric substances  arsenic  accumulation  transformation
基金项目:国家自然科学基金项目(31770548);南京农业大学大学生创新创业训练计划项目(1813A17)
作者单位
李崇华,赵方慈,喻琪盛,陈杨沁,张春华,葛滢 1.南京农业大学资源与环境科学学院江苏 南京 2100952.南京农业大学生命科学实验中心江苏 南京 210095 
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中文摘要:
      为探明胞外聚合物(EPS)对微藻砷的富集和迁移的作用效果,采用1 mmol/L乙二胺四乙酸(EDTA)对莱茵衣藻EPS进行提取,测定EPS含量及其主要成分,运用LIVE/DEAD BacLight staining–LSCM观察去除和未去除EPS的莱茵衣藻的生长情况,并对藻细胞进行AsV处理(20 μg/L、处理6.0 h),比较有(无)EPS的莱茵衣藻对砷的吸收、吸附和转化的差异。结果表明:莱茵衣藻EPS总量为16.12 mg/g,其主要成分为多糖(89.96%),并含有少量的蛋白质(7.41%)和DNA(2.63%),EDTA提取EPS后没有细胞自溶现象产生,不影响微藻的生长;去除EPS后的莱茵衣藻对AsV的还原能力显著降低,且在6.0 h内对砷的吸收没有明显变化,但砷吸附量占富集的比例(43.03% ~ 66.23%)显著高于有EPS的砷吸附占比(10.68% ~ 31.90%),表明EPS在莱茵衣藻对砷的富集和形态转化中具有重要作用。
英文摘要:
      In order to explore the effects of extracellular polymeric substances (EPS) on the accumulation and migration of arsenic(As) in microalgae, EPS of Chlamydomonas reinhardtii were extracted with 1 mmol/L ethylenediaminetetraacetic acid(EDTA). The content and major components were determined and the cell viabilities after EPS removal were observed using LIVE/DEAD BacLight staining and laser confocal microscopy. The As absorption, adsorption and transformation by C. reinhardtii with and without EPS were compared under the AsV treatment(20 μg/L, 6.0 h). The results showed that the total EPS of C. reinhardtii was 16.12 mg/g, with primary component of polysaccharide(89.96%) and a small amount of protein (7.41%) and DNA (2.63%). The EDTA extraction did not cause cell lysis and did not affect the growth of C. reinhardtii. The AsV reduction by C. reinhardtii was significantly reduced after the removal of EPS. No significant change in the As absorption was found within 6.0 h, however, the ratio of As adsorption to accumulation of C. reinhardtii without EPS (43.03%-66.23%) was significantly higher than that with EPS(10.68%-31.90%), indicating that EPS may play an important role in the accumulation and transformation of arsenic by C. reinhardtii.
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