袁野,朱仕豪,凌志辉,曾敏,陆英,钟晓红.瓜蒌皮多酚纯化工艺及抗氧化活性[J].湖南农业大学学报:自然科学版,2019,45(4):.
瓜蒌皮多酚纯化工艺及抗氧化活性
  
DOI:
中文关键词:  瓜蒌皮  多酚  纯化工艺  大孔吸附树脂  抗氧化活性
英文关键词:pericarpium trichosanthis  polyphenol  purification process  macroporous resin  antioxidant activity
基金项目:湖南省重大科技成果转化项目(2014CK1005)
作者单位
袁野,朱仕豪,凌志辉,曾敏,陆英,钟晓红 湖南农业大学园艺园林学院湖南 长沙 410128 
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中文摘要:
      以瓜蒌皮为研究对象,采用大孔吸附树脂法纯化瓜蒌皮中的多酚类化合物,对纯化工艺参数进行筛选,对纯化产物的抗氧化活性进行检测。结果表明:AB–8大孔吸附树脂对瓜蒌皮多酚具有良好的吸附和解吸性能,最佳吸附工艺参数为提取液中多酚质量浓度0.60 mg/mL,料液pH值3.0,上样流速2 BV/h,上样体积4 BV;最佳洗脱工艺参数为洗脱液乙醇的体积分数60%,体积6 BV,洗脱流速2 BV/h;纯化后产品中多酚的含量达49.52%,较纯化前提高57倍;瓜蒌皮多酚纯化前后对DPPH+自由基清除作用的IC50分别为2.01 mg/mL和0.53 mg/mL,对ABTS+自由基清除作用的IC50分别为0.62 mg/mL和0.13 mg/mL,纯化后抗氧化活性增强了4~5倍。
英文摘要:
      In this study, we investigated the variables of purification process of polyphenolic compounds from pericarpium trichosanthis and these variable effects on the antioxidation activity of the purified products. The method to purify was macroporous adsorption resin. The results showed that AB-8 macroporous resin has the best adsorption and desorption performance. The optimized adsorption process variables should be at: the concentration of polyphenols of extract 0.6 mg/mL; pH 3.0, the flow rate of fluid 2 BV/h, the volume 4 BV. The optimized elution parameters included the eluting regent 60 % ethanol, eluent volume 6 BV, elution rate 2 BV/h. By use of the optimized process, the purified polyphenol content was 49.52%, 57 times higher than those purified from the conventional process. The antioxidant activity of purification product increased 4-5 times than those form the conventional method. The IC50 of DPPH+ free radical scavenging effect of the polyphenols in pericarpium trichosanthis before and after purification were 2.01 mg/mL and 0.53 mg/mL, respectively, while the IC50 of free radical scavenging effect on ABTS+ was 0.62 mg/mL and 0.13 mg/mL, respectively.
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