许佳瑶,王博文,李丹蕾.松材线虫BxPrx基因抗H2O2胁迫的转录模式及功能[J].湖南农业大学学报:自然科学版,2019,45(5):.
松材线虫BxPrx基因抗H2O2胁迫的转录模式及功能
  
DOI:
中文关键词:  松材线虫  过氧化物还原酶基因  H2O2胁迫
英文关键词:Bursaphelenchus xylophilus  peroxiredoxin gene  H2O2 stress
基金项目:中国安全局国家重点研发计划(2018YFC1200400);国家自然科学基金项目(31971656);教育部中央高校基本科研业务费专项(2572017PZ08)
作者单位
许佳瑶,王博文,李丹蕾 东北林业大学林学院黑龙江 哈尔滨 150040 
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中文摘要:
      为探究松材线虫(Bursaphelenchus xylophilus)响应H2O2胁迫的分子机制,利用荧光定量PCR测定松材线虫过氧化物还原酶基因BxPrx在不同浓度(2.5、5.0、7.5、10.0 mmol/L)H2O2 胁迫下的转录水平;利用RNA干扰技术,沉默松材线虫BxPrx基因,比较H2O2胁迫下BxPrx基因沉默组与非基因沉默组松材线虫存活率的差异。结果显示:松材线虫的存活率与H2O2胁迫浓度呈负相关;松材线虫BxPrx在H2O2胁迫下的转录水平均提高;在5.0、7.5、10.0 mmol/L H2O2胁迫下,经过BxPrx基因沉默处理的松材线虫存活率显著低于对照组。
英文摘要:
      In order to explore the molecular mechanism of oxidative stress resistance in pine wood nematode (Bursaphelenchus xylophilus), the transcription level of a peroxiredoxin gene BxPrx under different concentrations of H2O2 (2.5, 5.0, 7.5, 10.0 mmol/L) was studied by qRT-PCR. In addition, RNA interference was used to silence the BxPrx gene of pine wood nematode, and the survival rate of BxPrx gene silenced group and un-silenced group under different concentration of hydrogen peroxide treatment was compared. The results showed that the survival rate of pine wood nematode was negatively correlated with the concentration of H2O2. The transcription level of pine wood nematode BxPrx increased under various concentrations of H2O2 stress. The survival rate of pine wood nematode treated with BxPrx gene silencing was significantly lower than that of the control group under H2O2 stress at concentrations of 5.0, 7.5 and 10.0 mmol/L.
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