殷全玉,刘健豪,方明,王生才,刘国顺,肖艳松.高碳基肥配施菌剂对植烟土壤化学性质及微生物的影响[J].湖南农业大学学报:自然科学版,2019,45(5):.
高碳基肥配施菌剂对植烟土壤化学性质及微生物的影响
  
DOI:
中文关键词:  高碳基肥料  哈茨木霉  枯草芽孢杆菌  植烟土壤  理化性质  微生物群落
英文关键词:high-carbon fertilizer  Trichoderma harzianum  Bacillus subtilis  tobacco-growing soil  physicochemical properties  microbial community
基金项目:中国烟草总公司开发重大专项[110201101001(TS–01)
作者单位
殷全玉,刘健豪,方明,王生才,刘国顺,肖艳松 1.河南农业大学烟草学院河南 郑州4500022.湖南省烟草公司郴州市公司湖南 郴州 423000 
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中文摘要:
      2018年,在湖南省郴州市桂阳烟区,设置5种施肥处理:CK(常规施肥)、T1(常规+高碳基肥料)、T2(常规+高碳基肥料+枯草芽孢杆菌)、T3(常规+高碳基肥料+哈茨木霉)、T4(常规+高碳基肥料+枯草芽孢杆菌+哈茨木霉),测定植烟土壤全碳、全氮、速效磷、速效钾含量,使用高通量技术分析土壤微生物多样性及群落结构特征,探究高碳基肥料配施微生物菌剂对植烟土壤化学性质和微生物群落的影响。结果表明:1)与CK相比,T2、T3、T4的土壤全氮含量分别提高20%、28%、28%,均与CK存在显著差异,施高碳基肥料处理的土壤硝态氮含量分别提高48.70%、49.90%、89.18%、111.72%,均与CK存在显著差异;2)与CK相比,各处理细菌OTU数量分别降低8.17%、10.03%、17.40%、14.04%,其中T3、T4与CK间存在显著差异,T2和T3间细菌Chao1、ACE指数均存在显著差异,T1和T2间真菌Shannon、Simpson指数存在显著差异;3)与CK相比,T1对各优势菌门相对丰度的影响不显著,T2、T3的放线菌门相对丰度分别提高79.86%、41.88%,T3的硝化螺旋菌门相对丰度降低29.31%;T2的壶菌门相对丰度提高107.31%,T4的担子菌门相对丰度提高86.86%,均与CK差异显著,各处理对土壤微生物群落结构的影响不稳定,对细菌、真菌的影响效果不相同;4)相关性分析表明,土壤优势菌群相对丰度与全氮、铵态氮、速效钾含量呈显著相关,说明高碳基肥对土壤化学性质的改变影响了土壤优势菌群的丰度。
英文摘要:
      In 2018, five fertilization treatments were set up in Guiyang tobacco district, Chenzhou city, Hunan province: CK (conventional fertilizer), T1 (conventional + high carbon base fertilizer), T2 (conventional + high carbon base fertilizer + Bacillus subtilis), T3 (conventional + high carbon base fertilizer + Trichoderma harzianum), T4 (conventional + high carbon base fertilizer + Bacillus subtilis + Trichoderma harzianum). Soil total carbon, total nitrogen, available phosphorus, potassium and other chemical indicators were determined and high-throughput technology was used to analyze soil microbial diversity and community structure to explore the effects of high-carbon fertilizer combined with microbial agents on chemical properties and microbial community of tobacco-growing paddy soil. The results show that: 1) Compared with CK treatment, the soil total nitrogen content of T2, T3 and T4 treatments increased by 20%, 28% and 28% respectively, showing significant differences with CK treatment. Nitrate nitrogen content in soil treated with high carbon-based fertilizer increased by 48.70%, 49.90%, 89.18% and 111.72% respectively, which had significant difference with CK treatment. 2) Compared with CK treatment, the number of bacterial OTU decreased by 8.17%, 10.03%, 17.40% and 14.04% respectively, and there were significant differences in the number of bacterial OTU between T3, T4 and CK treatment. There were significant differences in bacterial Chao1 and ACE indices between T2 and T3, and significant differences in fungal Shannon and Simpson indices between T1 and T2. 3) Compared with CK treatment, T1 treatment with high carbon base fertilizer had no significant effect on the dominance and relative abundance of fungi. The relative abundance of Actinobacteria in T2 and T3 treatments were increased by 79.86% and 41.88%, respectively. The relative abundance of Nitrospirae was decreased by 29.31% in T3 treatment. The relative abundance of Chytridiomycota was increased by 107.31% in T2 treatment and the relative abundance of Basidiomycota increased by 86.86% in T4 treatment. There were significant differences between them and CK treatment. The effects of different treatments on soil microbial community structure were not stable, and the effects on bacteria and fungi were different. 4) The correlation analysis showed that there was a significant correlation between the content of total nitrogen, ammonium nitrogen and available potassium in the soil and the relative abundance of dominant soil microflora, which indicated that the change of soil chemical properties by high carbon base fertilizer affected the abundance of dominant soil microflora.
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