郑小壮,陈清华,陈文坚,郭照良.基于线粒体COⅠ基因序列的掌肢新米虾7个自然群体遗传多样性分析[J].湖南农业大学学报:自然科学版,2019,45(5):.
基于线粒体COⅠ基因序列的掌肢新米虾7个自然群体遗传多样性分析
  
DOI:
中文关键词:  掌肢新米虾  自然群体  COⅠ基因  遗传多样性  遗传结构
英文关键词:Neocaridina palmata  natural population  COⅠ gene  genetic diversity  genetic structure
基金项目:国家环保专项(PM–zx097–201812–244);2019年广东大学生科技创新培养专项(pdjh2019b0510);广东省动物分子设计与精准育种重点实验室项目(2019B030301010)
作者单位
郑小壮,陈清华,陈文坚,郭照良 1.佛山科学技术学院生命科学与工程学院广东 佛山 5282312.生态环境部华南环境科学研究所广东 广州 5105203.惠州市渔业研究推广中心广东 惠州 516002 
摘要点击次数: 45
全文下载次数: 21
中文摘要:
      采用DNA条形码技术,对采自广东清远、惠州、韶关、广州与广西河池、南宁、崇左的7个掌肢新米虾(Neocaridina palmata)野生群体的97个样本的线粒体COⅠ基因片段进行PCR扩增和测序分析,研究掌肢新米虾自然群体的遗传多样性及遗传结构。结果表明:掌肢新米虾在624 bp的COⅠ基因序列中A、T、C、G碱基的平均含量分别为26.98%、33.17%、20.99%、18.85%,AT含量(60.15%)高于CG含量(39.84%),表现出较强的AT偏倚性;基于COⅠ基因序列的总群体中共检测到4个核苷酸变异位点,定义了5种单倍型,平均核苷酸差异数(K)、单倍型多样性指数(Hd)以及核苷酸多样性指数(Pi)分别为0.611、(0.557±0.025)、(0.000 98±0.000 07),呈现出高单倍型多样性和低核苷酸多样性;7个自然群体的群体内遗传距离为0.000~0.001,群体间的遗传距离为0.000~0.002;分子方差分析(AMOVA)和遗传分化系数(Fst)结果揭示,掌肢新米虾的遗传变异主要来自于群体间;中性检验和核苷酸错配分布表明,掌肢新米虾7个自然群体遵循群体扩张模式,发生了历史扩张事件;系统发育分析显示,广东4群体与广西3群体形成姐妹支,表明不同地理区域具有一定的种群特征,可作为单独的管理单位进行保护,Hap1与Hap4分别是广东4群体与广西3群体和惠州群体的共享单倍型,其余单倍型为各群体所独享。
英文摘要:
      In order to study the genetic diversity and genetic structure of the natural population of Neocaridina palmata, DNA barcode technology was used to amplify, sequence and analyz of mitochondrial COⅠ gene fragments from 97 samples in 7 wild populations(Qingyuan, Huizhou, Shaoguan, Guangzhou, Hechi, Nanning and Chongzuo). The results showed that the average content of A, T, C and G bases in the 624 bp COⅠ gene sequence of Neocaridina palmata in the Guangdong and Guangxi region were 26.98%, 33.17%, 20.99% and 18.85%, respectively, showing a greater preference for A and T base with the AT content(60.15%) and GC conent(39.84%). A total of 4 polymorphic sites and 5 haplotypes were identified in the total population based on COⅠ gene sequence. Moreover, the average number of nucleotide differences(K), haplotype diversity(Hd) and nucleotide diversity(Pi) were 0.611, (0.557±0.025) and (0.000 98± 0.000 07), respectively, indicating high haplotype diversity and low nucleotide diversity. The genetic distance within the population of 7 natural populations was 0.000–0.001, and the genetic distance between populations was 0.000–0.002. Analysis of molecular variance(AMOVA) and genetic fixationindex(Fst) revealed that the genetic variance of N. palmata was mainly from the population. The neutral test and nucleotide mismatch distribution indicated that the seven N. palmata did follow the population expansion pattern and had ever experienced population expansion. Phylogenetic analysis showed that the Guangdong 4 population and the Guangxi 3 population formed a sister branch, indicating that different geographical regions have certain their own population characteristics and can be used to protect separate management units. In addition, Hap1 and Hap4 were shared haplotypes of Guangdong 4 populations, Guangxi 3 populations and Huizhou population, respectively, and the remaining haplotypes are exclusive to each population.
查看全文  查看/发表评论  下载PDF阅读器
关闭
Copyright © 2008 《湖南农业大学学报》自然科学版 地址:湖南 长沙市 芙蓉区 
邮编:410128 电话:0731-84638380 0731-84618035 0731-84617599 0731-84673730 E-mail: zkb4618035@hunau.net  京ICP备09084417
分享按钮